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The pipette solution for whole-cell recordings was modified to include an intracellular dye (Alexa Fluor 350 hydrazide dye) and contained: 120 mM K-gluconate, 10 mM KCl, 10 mM HEPES, 5 mM EGTA, 1 mM CaCl 2 , 1 mM MgCl 2 and 2 mM MgATP, and 0.03 mM Alexa Fluor 350 hydrazide dye (pH 7.3)

Zhou J, Wang G, Chen Y, Wang H, Hua Y, Cai Z

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doi: 10.1016/j.immuni.2015.02.002 34 ZhangQRaoofMChenYSumiYSursalTJungerWet al

J Biol Chem 2003;278:25738-44

Understanding the molecular determinants of resistance and using this to guide rational combination therapy approaches is fundamental to improve clinical outcomes for patients with cancer